Effect of fasting on PPARg and AMPK activity in adipocytes
نویسندگان
چکیده
We investigated the effects of fasting on gene expression and intracellular signals regulating energy metabolism in adipose tissue. Following fasting for 15 h or 39 h, epididymal fat pads were isolated from Wistar rats. PPARg mRNA levels decreased in the adipose tissues isolated from rats fasted for 39 h, whereas adipocyte lipid-binding protein (aP2) and lipoprotein lipase (LPL) mRNA levels increased. Overnight fasting increased the AMP/ATP ratio and AMP-activated protein kinase (AMPK) in adipose tissue, but not in muscle or liver tissue. In addition, the effect of 5-aminoimidazole-4-carboxyamide-ribonucleoside (AICAR) on PPARg expression in primary cultured adipocytes was investigated. AICAR reduced PPARg mRNA levels but increased aP2 and LPL mRNA levels. Thus, fasting-induced AMPK activation may affect on the regulation of gene expression in adipocytes. # 2008 Elsevier Ireland Ltd. All rights reserved. avai lable at www.sc iencedi rec t .com journal homepage: www.e lsev ier .com/ locate /d iabres
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